"

From 2010.igem.org

Contents 1 Colony PCR of Genomic DNA 1.1 Aim: 1.2 Materials: 1.3 Protocol:

Colony PCR of Genomic DNA

Aim:

To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.

Materials:

• Pipette
• Microfuge
• Microtubes
• Distilled H2O
• Nucleotide DNTP
• 5x GoTaq buffer
• Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
• Forward and reverse primers

Protocol:

1. Materials added according to Colony PCR on Protocol list.
2. Melting temperature, Tm used for Anneal step is 59°C.