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JULY: WEEK 3
July, 12th
Phasins PhaP1 and PhaP2 were sequenced, but none of them has a clear chromatogram, so we decided to sequence the PCR results.
 Gel run/cut of phasins amplified via PCR Phasins were gel run/cut and the samples were prepared to be sequenced.
Inoculum of
- <partinfo>BBa_J23101</partinfo>
- <partinfo>BBa_J23105</partinfo>
- <partinfo>BBa_J23106</partinfo>
for tomorrow ligations.
July, 13th
July, 14th
 Quality control for HELPER and CRIM plasmids July, 15th
 Quality control for pBHR68 and CRIM plasmids July, 16th
 Screening ligations I11-I16 |  Screening ligations I17-I19, I7/I8/I10-4C5 |
Since it's impossible to isolate the CRIM plasmid <partinfo>BBa_J72007</partinfo> we created a pseudo CRIM plasmid to check that not pir strains aren't able to propagate it.
We called it "THE RING" (RING) and we built it from our I5 (Cm resistance - <partinfo>BBa_P1004</partinfo> - + TT - <partinfo>BBa_B0015</partinfo> - + R6K ori - <partinfo>BBa_J61001</partinfo>) in <partinfo>pSB1A2</partinfo>:
- Digestion of I5 XbaI-PstI
- Gel extraction
- Quantification with Nanodrop (15ng/ul)
- Ligation of X and P sites each other (used 2ul of DNA)
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