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JULY: WEEK 2
July, 5th
LB agar plates prepared:
- LB+Amp 100 (500ml)
- LB+Cm 34 (500ml)
- LB (250ml)
- LB+Cm 12.5 (250ml)
July, 6th
July, 7th
July, 8th
Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells
- MG1655
- BW25141
- BW25142
- BW53474
- DH5-alpha (as control)
to verify the transformation efficiency.
Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.
July, 9th
We checked the presence of colonies in plates.
All plates showed red colonies!
 MG1655 plate (with satellite colonies) | |
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We calculated (thanks Nicolò) efficiency as #colonies/ug DNA plated
| Strain | #colonies | Efficiency
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| MG1655 | 379 | ~10^5
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| BW25141 | 1431 | ~10^6
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| BW25142 | 2374 | ~10^6
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| BW53474 | 9984 | ~10^7
(very difficult to count correctly)
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| DH5alpha | 7475
(only 100ul of cells plated) | ~ 10^7
(very difficult to count correctly,
previous tests showed an efficiency of ~10^8)
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