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From 2010.igem.org

Alkane Degradation

With a number of yesterday's colonies a digestion was done to check if they contain the desired insert. The table below describes the digestion procedure, as well as the gel lane description.

#	Contents	Enzyme 1	Enzyme 2	Buffer	BSA	Expected size(s) (bp)
1	Smartladder (5μl)					n/a
2	007T (2)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	1341, 2455
3	008T (4)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	276, 2455
4	009T (6)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	285, 2455
5	009T (7)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	285, 2455
6	010T (9)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	1382, 2455
7	017T (14)	0.5 μl EcoRI	0.5 μl PstI	3 (Biolabs)	no	855, 2455
8	Empty
9	007T (2)	1 μl PstI	1 μl PvuII	H (Boehringer)	no	2529,983,251
10	008T (4)	1 μl AatII	0.5 μl KpnI	A (Boehringer)	1 μl	271, 2427
11	009T (6)	0.5 μl KpnI	1 μl NruI	B (Boehringer)	1 μl	598, 2109
12	009T (7)	0.5 μl KpnI	1 μl NruI	B (Boehringer)	1 μl	598, 2109
13	010T (9)	1 μl EarI	0.5 μl PstI	1 (Biolabs)	no	1075, 2729
14	017T (14)	0.5 μl AdhI	0.5 μl SpeI	4 (Biolabs)	no	428, 2849
15	EZ load (ladder)					n/a