Team:TU Delft/22 July 2010 content
From 2010.igem.org
Alkane degradation
There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.
# | Description | Expected length (bp) |
1 | [SmartLadder] (5 μL) | n/a |
2-4 | 007T | 1616 |
5-6 | 008T | 551 |
7-9 | 009T | 560 |
10-12 | 010T | 1657 |
13-15 | 017T | 1130 |
16 | 018T | 1874 |
17 | Red colony | n/a |
A number of colonies look promising! To check if they really are the BioBricks we want, tomorrow we will cut them with various restriction enzymes and analyze the digestion products on gel.