Team:TU Delft/22 July 2010 content

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Alkane degradation

There were some colonies on Tuesday's plates! We had left the plates @ 37°C yesterday after having seen that there were no colonies. When checking this morning on all plates (except the negative control) there were a few colonies! (2-50 colonies). Chances are it's not what we're looking for, but maybe they are good transformants... to check we will do a colony PCR.

1% Agarose gel of SC PCR samples
# Description Expected length (bp)
1 [SmartLadder] (5 μL) n/a
2-4 007T 1616
5-6 008T 551
7-9 009T 560
10-12 010T 1657
13-15 017T 1130
16 018T 1874
17 Red colony n/a
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