Team:Stanford/Research
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Project Goals
Digital Sensor
- Accurately report whether the concentrations of input chemicals lie at, above, or below a given ratio
- Allow as little expression as possible at the detection ratio, and as much as possible at all other ratios
Analog Sensor
- Accurately report over a large range of input ratios
- Achieve a linear correlation between output protein concentration and detection ratio
- Have a low basal level of expression
Both Sensors
- Allow future users to change output proteins and input promoters without disrupting the sensor's internal machinery
- Have both sensors be orthogonal to the host system to avoid cross-talk
Important Parameters
Digital Sensor
- Range of possible ratios
- Background expression level
- Of both proteins at ratio
- Of either protein when it shouldn't be being expressed
- Response curve
- Response time
Analog Sensor
- Range of possible ratios
- Background expression level (no kinase-inducing chemical added)
- Phosphotase specificity
- Response curve
- Response time