Team:Cambridge/References/ProjectBioluminescence/Luciferase

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Project Description | Luciferase | Luciferin Recovery | Light Output | Experiments | Modelling

Photinus Pyralis (Firefly Luciferase)

EPIC luciferase

Bacterial Bioluminescence

Vibrio Fischeri
Vibrio Harveyi
Genetics of luxCDABE (adapted from wikipedia)
[http://parts.mit.edu/wiki/index.php/Lux_operon| 2006 attempts at BioBricking the lux operon]
Notes
[http://partsregistry.org/Lux The lux wiki from parts registry]

[http://www.ncbi.nlm.nih.gov/nuccore/AF170104.1?report=graph&log$=seqview NCBI Lux operon sequence]
[http://partsregistry.org/Part:BBa_G10001 parts registry lux operon] -currently waiting on an email to see how useful/available this part is
IMPORTANT INFO

Plasmid experiment

[http://departments.kings.edu/biology/lux/bacterial.html Vibrio plasmid experiment] -possible source of lux operon

File:4449975.pdf

[http://departments.kings.edu/biology/lux/index.html] - Transformation protocol for students from basics - includes several plasmids

Plan
- set plasmid 555 into a BioBrick
- replace the promoter? sensitivity tuners?

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