Restriction digests

From 2010.igem.org

To cut DNA at specific base sequences using restriction enzymes

Materials

The following are required to make a 30 µl solution:

• 1 µl of restriction enzymes in total
• 3 µl of 10x buffer
• remainder is DNA/water

Notes

• No more than 10% of enzyme - solution contains glycerol which inhibits reaction
• 10x buffer must be diluted to 1x i.e. 10% final volume

Methods

1. Load desired quantities of solution into microfuge tube
2. Incubate at 37°C for 3 hours.