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JULY: WEEK 4
July, 19th
Trasformation of RING into:
- BW25141 (pir+)
- BW25142 (pir116)
- BW23474 (pir116)
- DH5alpha
- MG1655
Cultures were plated on:
- BW2514: Cm 34ug/ml
- BW25142: Cm 34ug/ml
- BW234741: Cm 34ug/ml
- DH5alpha: Cm 12,5ug/ml
- MG1655: Cm 12,5ug/ml
July, 20th
Results for plates incubated ON, 37°:
- BW23474 (pir116): showed colonies
- BW25141 (pir+): showed colonies
- BW25142 (pir116): showed colonies
- DH5alpha: didn't show colonies
- MG1655: didn't show colonies (even if there are a very few colonies that we suppose integrated the resistance of RING to survive - or the plate antibiotic wasn't homogeneous).
Single colonies were picked from plates and grown in LB+Cm at proper concentration. MG1655 colonies were let grow in LB+Cm to check if they integrated the Cm resistance of RING.
July, 21st
July, 22nd
July, 23rd
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