Team:Stanford/Notebook/Lab Work/Week 3

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries

7/5 Monday

Planning Meeting Notes

Agenda

• Design Updates
  • Analog Comparator (Chris & Alex)
  • Redundancy between the two proposals
  • Degree of phosporylation
  • Dynamic range and mechanisms

• • Digital Comparator (Francisco & Karina)
  • RSID; standardizing the design
  • Introducing a PoPs signal output

• Characteristics between the two Comparators
  • What makes the two different?

• Applications
  • Vaginal Infections/Preterm Birth

• is it really a ratio?
• Other ideas: Targeting Cancer Cells, oscillators

• Administrative Details
  • Ordering Stuff: Oligos ordering sheet, Digestion and Gel Extraction Kits?
  • Stipend?
  • Sponsorship Updates
  • MDV Presentation

Goals For the Week

• Chris & Alex
  • need to make 50x TAE
  • complete plasmids as far can get w/o kinase and phosphotase
  • order DNA sometime this week
• Francisco & Karina
  • Run orginal and new designs by Christina
  • design standard sRNA designs and place order by the end of the week

7/6 Tuesday

Greg:

• During weekly meeting got up to speed with what the team’s been doing while I was away
• Used heat-shock transformation protocol for DH5alpha to transform with the following genetic material, left to culture overnight:

7/8 Thursday

7/9 Friday