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From 2010.igem.org

Contents 1 Lab work 1.1 Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing 1.2 Characterization of Anderson RBS sequences 1.2.1 Fluorescence measurements Attempt #2 1.2.2 Assembly of reference construct

Lab work

Ordered DNA + Solvent Tolerance and Hydrocarbon Sensing

The colony PCR of yesterday was put on1% agarose gel

We harvested the 1 mL bacterial cells of possible correct transformations. The pellets were stored at -20 °C. We used 3 mL of the bacterial cells to make -80 °C stocks.

Characterization of Anderson RBS sequences

Fluorescence measurements Attempt #2

Data analysis to follow shortly (good stuff!)

Assembly of reference construct

The Birnboim method was used to isolate J23100 and I13522 (both in pSB1A2) from the o/n 5 mL LB cultures. The following concentrations of plasmid were obtained:

BioBrick	Concentration (ng/μL)
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23100 J23100]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13522 I13522]