"

From 2010.igem.org

Christopher's Lab Notebook for 7/13/10

• Run Gels (0.8%) of Restriction Digests:

 1M, 2M, 2J (X/P)
 F2620 (E/S)
 3C5 (E/P)
 I0500 (E/S)

Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation.