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From 2010.igem.org

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Plates

L.B.O.

24-06-10

Creation of "L.B.O.": a deodorant stick of LB agar

All of the following steps were performed in a safety cabinet under sterile conditions.

1) disassembled a Degree deodorant stick and soaked in ethanol

2) removed the raising platform and covered it with Parafilm

3) coated the insides of the tube with mineral oil

5) removed platform and poured LB agar into the base of the stick, waited until it solidified

6) the Parafilmed platform was put back into the stick and lowered maximally

7) LB agar was poured on top of the platform, until the tube was full, waited until it solidifed

8) The top of the LB agar was sliced off with an ethanol-sterilized knife

9) 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate

10) L.B.O. stick was incubated overnight at 37C

Observations:

- although the knife cut through the LB agar easily, it was difficult to produce a completely flat surface

- solidified LB agar was effectively raised and lowered using the dial

- after incubation, a bacterial lawn was observed and a distinct E.coli smell was present

29-06-10

1) Approximately 5mm was sliced off the top of the used LB agar with an ethanol-sterilized knife

2) Streaked green colony from a plate of Cambridge parts

3) L.B.O. stick was incubated overnight at 37C

Observations:

- dark green streak and individual colonies present

- distinct E.coli smell