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From 2010.igem.org

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Plates

L.B.O.

24-06-10

Creation of "L.B.O.": a deodorant stick of LB agar

All of the following steps were performed in a safety cabinet under sterile conditions.

1μL disassembled a Degree deodorant stick and soaked in ethanol

2μL covered the raising platform with Parafilm

3μL coated the insides of the tube with mineral oil

5μL removed platform and poured LB agar into the base of the stick, waited until it solidified

6μL the Parafilmed platform was put back into the stick and lowered maximally

7μL LB agar was poured on top of the platform, until the tube was full, waited until it solidifed

8μL The top of the LB agar was sliced off with an ethanol-sterilized knife

9μL 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate

10μL L.B.O. stick was incubated overnight at 37C