Team:USTC

When amazed that life is a box of chocolate, we basically refer to sweets. However this year we USTC iGEM 2010 Team switch our attention to the unadorned plain box. Our expectation is to construct a Host-cell free Reaction Chamber using Synthetic Biology methods.

In this project, we will engineer E.coil to construct a Host-cell-free Reaction Chamber in three continuous steps. First, we will utilize our bacteria to construct a internal empty BMC by cloning essential pdu shell gene into bacteria. Second, we endeavor with rationale to architect different signal peptides to deliver target enzyme into BMC. Last, we will attempt to improve the traditional purification methods of BMC to enhance the yield of the products.

The advantages of our Host-cell-free Reaction Chamber is obvious. This Chamber could on one hand reduce towards host cells the toxicity yielded from the pathway intermediates, on the other hand subside the influence the host shedding on the synthesis of product, where is the meaning of Host-cell-free coming from. Additionally, Reaction Chamber may enhances the efficiency of the enzyme through the principle of substrates channelling(). By achieving all these goals we wish we can construct a robust production line in E.coli.

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)