Team:EPF Lausanne/FirstWeek

Revision as of 13:42, 16 July 2010 by Stevarri (Talk | contribs)

Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C
Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X
Run a gel for the PCR -> failed (mix of two different kits)
Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar)
Learned to autoclave
Restarted PCR (Asaia ORI + primers)
Plated Asaia (O/N culture)
Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out)
Transformation of E.coli DB3.1 with pUC19 to check its competence
Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet)
E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate)
Ordered material
Wiki brainstorming
Protocols printing and organization

Run a gel for the PCR of the previous day -> worked
Purification of PCR’s product -> ok
Preparation of the BBα_151020 (streak out)
Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
Look at Asaia with microscope -> we have cells (pictures)
Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
Plated Asaia to recover some WT (streak out)
Preparation of Asaia Culture (for Lemaître experiment) with Kan
Protocol LateX template
O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
Learning HTML for the wiki
Text for the project presentation due to iGem
Writing Protocols of basic procedures for the wiki page
Maxi-prep of Asaia + Kan

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