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From 2010.igem.org

• [http://mic.sgmjournals.org/cgi/reprint/155/8/2676.pdf The role of FIS in the Rcd checkpoint and stable maintenance of plasmid ColE1] ie good as the toggle control thingy
• [http://www3.interscience.wiley.com/cgi-bin/fulltext/119127730/PDFSTART Timing, self-control and a sense of direction are the secrets of multicopy plasmid stability]
• [http://aem.asm.org/cgi/reprint/65/6/2710.pdf The quiescent-cell expression system for protein synthesis in Escherichia coli]
• [http://apps.isiknowledge.com/full_record.do?product=UA&search_mode=GeneralSearch&qid=12&SID=W11BA3C7bKeHC5PbfoE&page=1&doc=1&colname=WOS ColE1 multimer formation triggers inhibition of Escherichia coli cell division.]
• [http://books.google.com/books?hl=en&lr=&id=OKn60-HZisUC&oi=fnd&pg=PA19&dq=hn-s+nucleoid+bacillus&ots=rstjtFpQ2Z&sig=15uulXPnHz-yyXMvMKRrTXcLk3U#v=onepage&q=h-ns&f=false book about bac genomes]
• [http://www.faqs.org/patents/app/20090004700 D. Summers' Patent Application for Chem. Induction of Quiescence in Bacteria]

Literally no documentation of h-ns and rcd in bacillus.

If we do quiescence, will have to be in e coli with bacillus as a side project. Have to get over IP issues with ucam + e coli. Implementation in ecoli will be fairly easy as it is a working system - all we need to do is brick it, and get it working.

Input from James

• Has to be done in E.Coli, will not work in non Coliform bacteria such as Bacilli.
• Switch on cell division would be very useful, however there are IP issues which must be discussed with David Summers.
• Another constraint is that liquid broth not agar must be used.
• Nobody has looked at it from a microfluidics point of view.
• When done before, the lambda phage takes 3 hours to initiate quiescence after the temperature change.
• The Lambda phage is the most stable to have an off switch, it does not express itself when needed (?)
• The RNA has a very long half life, it would be hard to strip away the system and put it under a new promotor, but, if possible would be extremely useful