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Team:Purdue/Notebook
From 2010.igem.org
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Notebook
6/16/10
Prepared nine pots of wildtype (Col-0) A. thaliana and moved to cold storage for stratification. Pots intended for use in Agrobacterium flower-dip. Seeds were sewn in soilless media and the pot was covered with a wide meshing to retain the soil.
Prepared proliferative media for GBAM1 glioblastoma cell line. Began culturing cells (n = 14).
6/19/10
Divided GBAM1 cells into media for proliferation and differentiation.
6/21/10
Transfered pots from cold storage to greenhouse. Prepared 0.5 MS agar media and sterilized seeds for sterile growth, to be sewn on 6/23. Seeds left in dilute agar solution in fridge to stratify.
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Replenished GBAM1 media. Selected cells for lactate flux sensing.
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===6/23/10===
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Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify.
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===6/24/10===
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Seeds sown onto agar. Five large culture plates and two small plates were then transfered into horticulture growth chamber. Seeds in soil media forecasted to germinate by 6/26.
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Replenished GBAM1 media. Selected cells for oxygen flux sensing.
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===6/25/10===
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A. thaliana expirement mapping: Testing low oxygen response in WT and Transgenic using atmospheric chamber in Purdue Agronomy department. Using sensors to measure ethanol, oxygen, auxin response in control and in hypoxic conditions.
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===6/25/10===
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Communicating with IU iGEM team concerning synergies including arabidopsis transformation workshop.
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Communicating with Naperville, Ill high school student as part of community outreach experience.
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Planning Purdue University campus poster session to involve public in forum discussion on genetic engineering.
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===6/28/10===
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Sterilized seeds for plates. Potted seeds on soil, placed in cold room.
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===6/29/10===
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Transformation of E. coli failed.
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===7/1/10===
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Poured agar. Placed new plants onto sterile plates. Placed in growth room. Placed potted plants in mist room.
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===7/6/10===
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Successful growth of all plants noted. One small mold colony present in plate.
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===7/12/10===
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Transformation of E. coli: attempt
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===7/13/10===
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Transformation of E. coli: Success
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