Team:TU Delft/9 May 2010 content

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Session 11

First meeting after have chosen our subject.

Made a preliminary planning:

Today, team division and general discussion on how to approach things
Next meeting: Preliminary plan on general approach per group, possible questions for the whole group, and a general discussion. When making the lab-plans, also incorporate deadlines at which goals must be reached.
Following, weekly meetings to keep everyone up to date?
End of May: Meeting with the professors / staff of kluyver etc in which each group’s plan will be presented, and we will ask the people attending to give advise / comments / remarks
June: Lab work!

As already mentioned, our subject can be divided into 4 subparts, but each subpart will need team members as well as an initial plan of action.

Team members: Hugo, Mathias & Kira

Focus on alkenes
Paper by Rojo: 3 main enzymes, Make different strains for each type of alkene degradation (different enzymes for different lengths), Gene’s are in a cluster, so design a primer for this cluster,
Using GC for measuring the alkenes?
Hydrocarbon Resistance: Paper by Lazaroaie => This is phase 2! First start with a “normal” strain under mild conditions – check how high the normal resistance is?
Drug efflux pump will create Drug resistance in E.coli (ethical subject!), Pseudomonas & E.coli membranes are pretty similar according to Hugo