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From 2010.igem.org

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In brief, this year's Berkeley 2010 iGEM team accomplished the following:

• A foundational advance in protein and DNA payload delivery to lower metazoans, opening the door to genetic modification of potentially very useful and previously intractable organisms using synthetic biology.
• An automated, user-friendly, forward-thinking approach to biosafety implemented in the software program Clotho, improving human practice in synthetic biology.
• Developed and deployed the new core of Clotho 2.0 as an App platform for synthetic biology tools

Requirements

Bronze Medal

• Register the team, have a great summer, and have fun attending the Jamboree.
• Create and share a Description of the team's project via the iGEM wiki.
• Present a Poster and Talk at the iGEM Jamboree.
• Enter information detailing at least one new standard BioBrick [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Berkeley Part or Device] in the Registry of Parts

Silver Medal

• Demonstrate that at least one Part or Device of your own design and construction works as expected. (See Self-Lysis and Vesicle-Buster)
• Characterize the operation of at least one new BioBrick [http://partsregistry.org/wiki/index.php?title=Part:BBa_K426020 Part or Device] and enter this information on the Parts or Device page via the Registry of Parts.

Gold Medal

• Characterize or [http://partsregistry.org/wiki/index.php?title=Part:BBa_K426020 improve] an [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112808 existing] BioBrick Part or Device and enter this information back on the Registry.
• Outline and detail a new approach to an issue of Human Practice in synthetic biology as it relates to your project, such as safety, security, ethics, or ownership, sharing, and innovation.

Bronze Mousepad

• Registered the team, had a great summer, and had fun attending the Jamboree.
• Created and share a Description of the team's project via the iGEM wiki.
• Present a Poster and Talk at the iGEM Jamboree.
• Develop and make available via the Registry an open source software tool that supports synthetic biology based on BioBrick standard biological parts. Source code available from sourceforge (http://sourceforge.net/projects/clothocad/). Though we highly recommend downloading one of the more user-friendly distributables from the clothocad.org website or the source using subversion from sourceforge, a zip file containing the source code can also be downloaded here.

Silver Mousepad

• Provided a detailed, draft specification for the next version of your software tool, or a second, distinct software tools project

Gold Mousepad

• Outlined and detailed a new technical standard for managing biosafety (Human Practices) in synthetic biology based on a standardized data model

Attributions

ChoaChoa's Delivery Service is composed of 4 undergraduate wetlab students, 2 undergraduate computational students, 2 graduate student advisors, 2 faculty advisors, and administrative support.

Our wetlab group of Christoph Neyer, Amy Kristofferson, Daniela Mehech, Conor McClune, and Tahoura Samad built the Choano payload delivery devices, transposase devices, developed and performed all assays associated with this project. Ben Bubenheim and Bing Xia built the infrastructure for Clotho's API, wrote the Datum layer, worked out the Netbeans and Hibernate platforms, the Biosafety framework, the Algorithm/Actor framework, and several Apps including Sequence Checker, BBAligner, StringAppend Algorithm, and Groovy Scripter. Tim Hsiau was the ringleader of the iGEM team providing training, advice, technical and moral support. Jin Huh provided parts derived from his mammalian payload delivery project which yielded many of the basic parts used in the project in addition to parts used from Berkeley iGEM 2008. Chris Anderson and Terry Johnson provided lab space, reagents, consumables, moral support, graphics, guidance, and training to the team. Chris Anderson wrote many of the Apps not mentioned here included within the distributed version of Clotho. The specific data model of the Clotho API was determined through many conversations over the past two years between Ben, Bing, Josh Kittleson, Douglas Densmore, Tim Ham, Michal Galdzicki, and Chris Anderson in collaboratin with the broader SBOL standards community. The new clothocad.org website was provided with support by Autodesk with advice from Carlos Olguin and produced by Noble Studios. The UC Berkeley iGEM is selected by the Berkeley iGEM Advising Group composed of Adam Arkin, Jay Keasling, Chris Anderson, Terry Johnson, and Susan Marqusee. We are blessed to receive administrative support from Kate Spohr.