Team:METU Turkey/Results Discussion/Glycerol stock preparation

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MATERIALS


- SOB/LB Media
- Glycerol (100%)
- Sterile Milli-Q dH2O
- Liquid nitrogen



CHECK-LIST PROCEDURE


- Choose colony put into 5 mL of LB medium in 15 mL falcon.
- Incubate at 37 C @ 240 rpm not longer than 12-14 h.
- Centrifuge 5000 rpm 5min.
- Discard 4 mL solution from falcon.
- Add 176 uL glycerol into falcon to 15% final concentration.
- Vortex!
- Aliquot 150 uL samples.
- Quick-freeze in liquid nitrogen
- Place in -80 C deep freeze

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