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From 2010.igem.org

Emulsifier

By Pieter

Today the digestion products from yesterday were run on gel to see whether the plasmids were cut in the right way.

Lane description

#	Description	Expected Length (bp)
1	Biorad EZ marker
2	Undigested plasmid backbone pSB1T3
3	Digested plasmid backbone [http://partsregistry.org/Part:pSB1T3 pSB1T3]	2500
4	Undigested AlnA
5	Digested AlnA	1107
6	Undigested OprG
7	Digested OprG	744
8	Undigested B0015
9	Digested [http://partsregistry.org/Part:BBa_B0015 B0015]	130
10	Undigested R0011 !
11	Digested [http://partsregistry.org/Part:BBa_R0011 R0011]	55
12	Undigested B0032
13	Digested [http://partsregistry.org/Part:BBa_B0032 B0032]	13
14	SmartLadder

! Sample not fully loaded on gel

The band position of the digestion products of AlnA and OprG correspond to their length. The other fragments (R0011, B0032 and B0015) probably run of the gel.

The ligation products that were incubated over night were transformed to TOP10 competent cells according to the protocol.