Team:UC Davis/notebook/onpathway.html

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ON pathway

Construct:

Step One

  • B0034 is ligated to both C0051 and E1010 (also known as RFP)

Step Two

  • RBS-RFP and F1610 are ligated together


Step Three

  • RBS-C0051 and RBS-RFP-F1610 are ligated together
  • This step is where we have discovered bizarre transcription!!!

Step Four

  • AFTER getting rid of the C0051 bar code, we have ligated the MODIFIED c0051 to the RBS-RFP-F1610.

Step Five

  • After ligating the piece to the I12006 promoter, we have now completed the ON switch!

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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