Team:Wisconsin-Madison/parts

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New Standards

Wisconsin-Madison Team has taken the initiative to improve maintenance of the Registry of Standard Parts with more user experience in the form of zip files! Each fill will be labeled by its corresponding part number in the registry (ie. BBa_K318501) and will contain the following:

  • Genbank including features and plasmid (.gbk)
  • Fwd and Reverse sequencing files (.ab1)
  • Gels from cloning and confirmation (.pdf)
  • All associated testing files and Results (.xls, .pdf, etc.)

Here is a copy of our submitted standard, BBF-RFC-64: Media:BBF_RFC_64.pdf

Enzyme Delivery

New DNA

Enzyme Delivery Testing Constructs
Part Number About Testing in... Zip File
<partinfo>BBa_k318512</partinfo> Regulatory - pH inducible promoter <partinfo>BBa_k318513</partinfo> here
<partinfo>BBa_k318514</partinfo> Gene - RamA protein can bind to cholic acid (bile salts) <partinfo>BBa_k318515</partinfo> Media:Zip_file_ramA.zip
<partinfo>BBa_k318516</partinfo> Regulatory - RamA/Bile-salt conjugate inducible promoter <partinfo>BBa_k318517</partinfo> Media:Zip_file_acrRA.zip


Testing Constructs

Enzyme Delivery Testing Constructs
Part Number Function Expression Type Zip File
<partinfo>BBa_k318500</partinfo> Produces Trascription Factor RcsB Inducible - IPTG here
<partinfo>BBa_k318501</partinfo> Produces Trascription Factor RcsA Inducible - IPTG here
<partinfo>BBa_k318502</partinfo> Produces Trascription Factor RcsA & RcsB Inducible - IPTG here
<partinfo>BBa_k318503</partinfo> Produces Trascription Factor YgiV - Blocks Biofilm Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318504</partinfo> Produces RfaI gene - WaaI Ligase Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318505</partinfo> Produces T.F. YgiV & RfaI gene Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318506</partinfo> Produces RFP (+)LuxR, (-)cILVA here
<partinfo>BBa_k318509</partinfo> Produces Repressor cI-LVA (degredation tag) Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318510</partinfo> Produces Repressor cI (no degredation tag) Inducible - IPTG DNA Not Sent
<partinfo>BBa_k318511</partinfo> Produces Activator LuxR Anhydrous Tetrycycline here
<partinfo>BBa_k318513</partinfo> Produces RFP pH here
<partinfo>BBa_k318515</partinfo> Produces RFP RamA/Bile-Salt Complex Media:Zip_file_acrRA.zip
<partinfo>BBa_k318517</partinfo> Produces RamA gene Bile Salt Media:Zip_file_ramA.zip


Final Constructs

Enzyme Delivery Final Constructs
Part Number Function Expression Type
Not Cloned Produces Trascription Factors RcsA & RcsB, and B-Gal Constitutive
Not Cloned A) Produces equivalent ratio of Activator(LuxR) and Repressor(cI-LVA) pH 2
<partinfo>BBa_k318507</partinfo> A) Activates Lysis once in the small intestine here (+)-LuxR, (-)-cILVA
<partinfo>BBa_k318519</partinfo> B) Activates lysis once in the small intestine Bile Salts


Encryption

Encryption Constructs
Part Number Description Notes Zip File
<partinfo>BBa_K318000</partinfo> Key Cassette - produces Cre and Hin recombinases Arabinose induces Cre expression, IPTG induces Hin expression Media:BBa_K318000_pSB1C3.zip
<partinfo>BBa_K318030</partinfo> Lock Cassette - prevents transcription unless "unlocked" Requires successive Hin and Cre recombination for constitutive expression of downstream gene(s) Media:BBa_K318030_pSB1C3.zip
<partinfo>BBa_K318521</partinfo> Lock Lysis Cassette - for use with iDIET Requires successive pH then bile sensing by the organism to initiate cellular lysis. Design phase only


Other Parts

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318010 BBa_K318010]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318011 BBa_K318011]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318012 BBa_K318012]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318013 BBa_K318013]
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K318014 BBa_K318014]