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From 2010.igem.org

October 5th

• Digests:

-R1062+RFP (SP)
-R0061+RFP (SP)

• Ligations:

-R1062+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
           -->J23108+LuxR (B)
           -->J23116+LuxR (B)

October 6th

We selected three colonies (a,b,c) from the plates and performed a colony PCR upon each collected sample.

• Colony PCR:

1. R1062+RFP + J23100+LuxR
2. R1062+RFP + J23108+LuxR
3. R1062+RFP + J23116+LuxR
4. R0063+RFP + J23100+LuxR
5. R0063+RFP + J23108+LuxR
6. R0063+RFP + J23116+LuxR

They were then run on a gel after being PCR'd.

• Gel Order:

- 1.a,b,c
- 2.a,b,c
- 3.a,b,c
- 4.a,b,c
- 5.a,b,c
- 6.a,b,c

• Grown In Flasks:

-1a (R1062+RFP+J23100+LuxR)
-2a (R1062+RFP+J23108+LuxR)
-3a (R1062+RFP+J23116+LuxR)
-4a (R0063+RFP+J23100+LuxR)
-5a (R0063+RFP+J23108+LuxR)
-6a (R0063+RFP+J23116+LuxR)

October 7th

Referencing the Notation used on October 6th.

• Minipreps:

-1a
-2a
-3a
-4a
-5a
-6a

• Lab Work

-Made Stock Solutions of the Minipreps
-Made SOB Media 
-Made Competent Cells
-Poured Agar Plates
-Sent Samples to Sequencing Center

We began to anticipate the Jamboree at this point and were diligently working to finish on time.

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