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From 2010.igem.org

October 19th

• Minipreps:

-J23116+K091146
-A3

There had been previous minipreps completed; however, the two above did not turn out correctly the first time. Because of this problem, they were miniprepped once more.

• Digested:

-K091107+J23116 (EX)
-K091107+J23108 (EX)
-K091146+J23108 (EX)
-K091146+J13116 (EX)
-AFP            (EX)
-TetCIR         (EX)
-LTC+J23116     (ES)
-LTC+J23108     (ES)

Note: There was protein contamination in K091146+J23116 after the digest.

• Gel Order:

-Ladder
-K091107+J23116 
-K091107+J23108 
-K091146+J23108 
-AFP            
-TetCIR         
-LTC+J23116     
-LTC+J23108     
-K091146+J13116

October 20-25th

At this point we had started to wrap up our work. We did not keep accurate records in our Lab Notebook; however, this is when we began sequencing our final parts and constructs.

As our sequences came back as expected, and needed, we did final ligations and transformations.

October 26th

FINAL DAY OF LAB WORK!!!

We placed the plasmids of the parts we were submitting to the registry into PCR strips and taped the first tube. These tubes were then placed into a large tube, sealed, and mailed to the Parts Registry to add. For a more comprehensive review of these submissions please see our [Parts Submitted to the Registry] page.

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