Sudoku

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Perspective Reference

Notebook

6/23

Transformation

2010 Kit plate1: 6N,1H,5D,17J,4H,18A

2010 Kit plate2: 24C,2K

6/24

Inoculation

2010 Kit plate1: 6N,1H,5D,17J,4H,18A

2010 Kit plate2: 24C,2K

(6/23 Transformation)

6/25

Miniprep

2010 Kit plate1: 6N,1H,5D,17J,4H,18A

2010 Kit plate2: 24C,2K

(6/24 Inoculation)

6/27

Enxyme digestion

1~7, 9 & 10 plasmid

1: 117.2 ng/uL -> 2.56 uL / SP

2: 59.3 ng/uL -> 5.06 uL / SP

3: 124.2 ng/uL -> 2.42 uL / XP

4: 114.5 ng/uL -> 2.62 uL / EX

5: 81.3 ng/uL -> 3.69 uL / SP

6: 101.7 ng/uL -> 2.95 uL / ES

7: 81.5 ng/uL -> 3.68 uL / EX

9: 85.4 ng/uL -> 3.51 uL / SP

10: 73.5 ng/uL -> 4.08 uL / SP

6/28

Gel extract

1~7, 9 & 10 plasmid (6/27 enzyme digestion)

6 -> failed, others -> succeeded

6/29

Cutcheck

EScut: 1~5, 7, 9 & 10 (6/27 enzyme digestion)

HindIII cut: 6

6/30

Ligation

2-3

2: 5uL, 3: 5uL, ligation mix: 10uL

(6/27 Enzyme digestion)

Transformation

2010 Kit plate1: 1N (TetA(C) (Backwards))

2010 Kit plate2: 22P (OmpA)

2-3 (6/30 Ligation)