ULB/23 August 2010
From 2010.igem.org
Module hydrogen
- We did the PCR of validation on all of our deletion candidates
- We continue the purification process for the deletion candidate
- We continue to try to obtain other deletion candidates
- The insertion of tdcE behind the RBS did not work. We try it again.
Module homologous recombination
- We did again minipreparations in order to exctrude the plasmid TOPO with the kan and cm resistance cassette
Module heavy metals detection
- The bacteria did not turn orange on the copper medium. We therefore decided to test separately the gene of the carotene and the copper sensitive promoter.
Other
- We tested by PCR our maxipreparations