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Talk:Team:IvyTech-South Bend/25 October 2010
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Revision as of 02:04, 28 October 2010 by Rchamberlin (Talk | contribs)
“THE BLUE GUY”
Today we will parity DNA from BlueGuy found on Friday. Also will do an assay testing our part against purified AHL, and pulling and ligating our Clonphenol backbone with our LacZ, & T900s for shipment.
First I will be pulling our DNA plasmid from the Bio Brick part SB1C3 From well 3A Plate 1 following protocol from p. 15. Then I will ligate and place with T9002 & LacZ which will be ligated from TCN resistant plasmid @ E, P plasmid will be ligated @ E,P as well.
Ligating Clonphenol backbone we used 8 ul DNA w/ 34.5 ul 18 ohm H2O and for part used 4 ul DNA w/ 38.5 ul 18 ohm H2O then followed the protocol from p. 53
--for JhullRchamberlin 13:19, 27 October 2010 (UTC)
for Dgarvey--Rchamberlin 02:04, 28 October 2010 (UTC)
