Team:Queens-Canada/9 August 2010

Miniprep

Hao, Thai, Nelson, Chris, Yuli

'eCFP::1C3, NpHR::1C3, pStr-1::1C3, pStr-220::1C3, pCeh-12::1C3, pOsm-10::1C3, pOdr-1::1C3, NpHR::1C3, pSrb-ChR2'::1C3, mCherry::1C3, pSB1C3

'pIC57ChR2'::1C3 did not pull through vacuum very well, may contain genomic DNA.

Gel and Digest

Chris and Thai

pOsm-10, pStr-220, pOdr-1

Cut with EcoRI and PstI.

Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V. Note: [4] pOsm-10 digest had <10 µL of liquid, most likely due to error during master mix loading.

Gel Electrophoresis

Chris and Thai

pOdr-1, NpHR, pStr-1, pCeh-12

Used 0.7 g agarose in 100 mL TBE. Gel run for 33 mins at 110 V.

Gel Electrophoresis

Chris and Thai

pCeh-12, pSrb-6, pIC576, eCFP

Used 0.7 g agarose in 100 mL TBE. Gel run for 26 mins at 110 V.

Microinjection

Hao

Practiced injections with Sur-5::GFP marker.