Team:Queens-Canada/26 July 2010

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July 26, 2010

Gel Electrophoresis

Yuli

pSB1A3, pSB1A2

Used 0.35 g agarose in 50 mL TBE. Gel run for 45 mins at 100 V. Well were deformed as comb was pulled out prematurely.

PCR

Thai

pSB1A3, pSB1C3, pSB1T3, pSB1A2

Program run using 9:1 FAST:HIFI blend with an extension time of 70 s and annealing temperature of 65 °C.

Heat Shock

Yuli

pSB1A3, pSB1T3, pSB1K3, pSB1C3

Microinjection

Hao

Practiced injections with Sur-5::GFP marker.

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