Team:Queens-Canada/23 June 2010

PCR

Steve and Yuli

pHsp-3

Program run using HIFI with an extension time of 45 s and annealing temperature of 58 °C.

2010 PCR Hao, Louis and Mary

pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP

Program run using HIFI with an extension time of 30 s and annealing temperature of 57 °C.

Gel Electrophoresis

Yuli and Nelson

pSip-1::eGFP, pRab-7::eGFP, pMec-7::eGFP, pHsp-3::eGFP

Used 0.4 g agarose in 65 mL TBE. Gel run for 40 mins at 100 V.

Heat Shock

Hao and Thai

pIC576, pIC517, pIC518

Plates stored in 37 °C room overnight.

DNA Rehydration

Hao and Thai

BBa_I13522

Heat Shock

Thai, Mike and Mary

BBa_I13522

Plates stored in 37 °C room overnight.

PCR

Thai, Louis and Mike

pHsp-3

Program run using HIFI with an extension time of 90 s and annealing temperature of 58 °C.

Microinjection

Hao and Yuli

Practiced injections with water.