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From 2010.igem.org

Chair: Da, Minutes: Younus, Comp

Contents 1 Cloning Strategies 1.1 5 parts to a Cloning Strategy: 1.2 Required Cloning Strategies 1.3 Allocation of team members to cloning strategies: 2 Wiki 2.1 Labbook 2.2 General: 3 20th/21st July - UK iGEM Gathering at Newcastle 3.1 Presentation: 3.1.1 Introduction 3.1.2 General Outlook 3.1.3 BioBrick Discussion 3.1.4 Whats next... 4 Minideadlines: 5 Action Points

Cloning Strategies

5 parts to a Cloning Strategy:

The cloning strategies for each biobrick should contain the following sections in this order:

1. Purpose and Justification
2. Modelling
3. Construction
4. Cloning and Integration
5. Testing and Characterising

Required Cloning Strategies

There are 8 cloning strategies which are required:

• SR1 - finished!
• YneA - biobrick nearly coming back - our most important biobrick!
• LacI - not sure how to test this biobrick
• 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
• 2 for Subtilin (immunity and production) - cloning strategies nearly finished
• RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!

Allocation of team members to cloning strategies:

• YneA - Phil, Rach, Deena, Younus
• Swarming (2) - Harsh, Alan, Steve, Da
• LacI - Phil, Younus, Rachel
• Subtilin (2) - Harsh, Alan, Steve, Deena
• RocF - Harsh, Alan, Steve, Da

Wiki

Labbook

Everyone needs to carry out the following protocol for each day in the lab

1. Aim
2. Materials and Methods
3. Procedure/Protocol
4. Results
5. Conclusion

Relevant photographs should be added and labelled

General:

• Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)

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20th/21st July - UK iGEM Gathering at Newcastle

Presentation:

Introduction

[2 mins] Rach, Deena, Jannetta

• Cover Page
• Picture of us standing up
• Introduction of our iGEM project - using BS to repair concrete

General Outlook

[5 mins] Harsh, Da, Phil

• Starting with bioreactor
• Transformation into spores
• Transportation
• Spraying on building
• Germination
• sensing crack + non-environmental kill switch
• swarming
• end of crack signalling = QS
• Filamentous cells
• CaCO3
• Glue
• Kill Switch

BioBrick Discussion

[8 mins altogether; 2 mins each] Alan, Younus, Steve

• Swarming
• Subtillin
• Filamentous cells - YneA
• CaCO3 - Urease

(5 mins remaining)

Whats next...

'Save the babies!!!'

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Minideadlines:

1. Cloning Strategies
   • YneA
   • One sequence for sfp primers and coding sequence ready (Goksel)
   • Subtilin just immunity

Action Points

1. Finish wiki updates by Sunday 18th (everyone)
2. Team page on Wiki:
   • Add Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili to list of advisors
   • Deena, Phil and Younus to write about yourself + pictures