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From 2010.igem.org

Lab work

The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks!

We were so excited; we immediately solved the DNA in water and performed a transformation with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic.

1. AlkB2 on 100 μg/mL Ampicillin 2. rubA3 on 100 μg/mL Ampicillin 3. rubA4 on 100 μg/mL Ampicillin 4. rubB on 50 μg/mL Kanamycin 5. ladA on 100 μg/mL Ampicillin 6. ADH on 100 μg/mL Ampicillin 7. ALDH on 50 μg/mL Kanamycin 8. bbc1 on 100 μg/mL Ampicillin 9. AlnA on 100 μg/mL Ampicillin 10. OprG on 100 μg/mL Ampicillin 11. PalkS1-2 on 100 μg/mL Ampicillin 12. PalkB on 100 μg/mL Ampicillin 13. P(CaiF) on 100 μg/mL Ampenicillin 14. AlkS on 50 μg/mL Kanamycin 15. PhPFD-α on 100 μg/mL Ampicillin 16. PhPFD-β on 100 μg/mL Ampicillin

Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples:

	Hexane (mL)	Tris Buffer pH 8 (mL)	10% SDS (mL)
B	1	1.1	0
100	1	1	0.1
50	1	1.05	0.05
25	1	1.075	0.025
10	1	1.09	0.01
5	1	1.095	0.005

Kampioenen!!!!

Today the Dutch team will play the semi-final of the World Cup! Hear us cheer! Stop sound