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From 2010.igem.org

Lab Work

We are a bit short handed in the lab this week, because most team members have exams. Considering the fine weather outside, it is not bad to have a slow week. All the transformations of yesterday containing BioBricks form the distribution plate gave colonies. We pick a colony of every plate and grow them overnight in 50 mL LB medium containing Ampicillin.

Today we cultivated Pseudomonas Putida GPO1 on different substrates:

1. E2 medium without carbon source

2. E2 medium + 1% LB

3. E2 medium + 10% LB

4. E2 medium + 1% octane

5. E2 medium + 10% octane

6. LB medium + 10% cyclohexane