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From 2010.igem.org

As summer project our experimental work began in August, after intensive brainstorming we finnaly decide

between two options.

*Inmunoresponse using Synthetic Biology.

*Cryobiology applied to plant crops.

After a selection process we chose the Cryobiology Project.

The final design of the expressions modules were as follow:

Next Notebook sheet is our reference for primers and ligations and for modules Assembly:

The AFP (Antifreeze Protein) was synthesized as follow:

Week #1

06th September - 10th September 2010

Monday

• We got plasmid PSB1C3 from the Biobrick plate.

• We prepared stock solutions of Cloramphenicol, Kanamicyn, Ampicilin.

• Some LB agar and 35ug/ml cloramphenicol plates made up .

• We al ready made quimio and electro-competent cells for transformation and stored in aliquots in containers vials at -80 degrees..

Tuesday

We transformed DH5α cells with PSB1C3.

Wednesday

We have not obtained transformats cells.

We checked out Cloramphenicol stock concentration and attempt again the transformation used PSB1C3.==

Thursday

The primers arrived

and we proceed to amplify the modules by PCR.

The amplification was ok and to quantify DNA concentrations

we had looking for a nano spectrophotometer.