BioBricks submitted

Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR

• BBa_K374008

This part contains the promoters: pR^Gifsy1 and pRM^Gifsy1. Downstream of the pRM^Gifsy1 promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM^Gifsy1 and a repressor of the pR^Gifsy1.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K374010 hence allowing expression of your inserted BioBrick.

This part has been characterized, for more information look here or in parts-registry, BBa_K374008

Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR

• BBa_K374009

This part contains the promoters: pR^Gifsy2 and pRM^Gifsy2. Downstream of the pRM^Gifsy2 promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM^Gifsy2 and a repressor of the pR^Gifsy2.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.

This part has been characterized, for more information look here or in parts-registry, BBa_K374009

Antirepressor AntO from Gifsy1 phage

• BBa_K374010

AntO inactivates the repressor from Gifsy1 phage, resulting in transcription from the pR^Gifsy1 promoter. AntO works by direct non-covalent binding to gogR and prevents its DNA binding activity.

This regulatory biobrick can be used in a biological circuit. It can de-repress the effect of the repressor from BBa_K374008 allowing expression from the pR^Gifsy1 promoter.

For more information look in parts-registry, BBa_K374010

NutRsite - N anti-terminator utilization site from Lamdba phage

• BBa_K374005

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda nutR site which together with the N-protein will suppress termination downstream regardless of what terminator is used.

This regulatory biobrick can be used in a biological circuit. It can supress any termination, if placed upstream of the terminator and applied together with BBa_K374006

For more information look in parts-registry, BBa_K374005

N anti-terminator gene from Lambda phage

• BBa_K374006

In lambda bacteriophage, gene expression is regulated by the suppression of transcription termination (antitermination) which is mediated by the lambda N protein that interacts with the nut site which is a cis-acting element. This part contains the lambda N gene which will suppress transcription termination downstream of the nutR site.

This regulatory biobrick can be used in a biological circuit. Together with BBa_K374005 , they will promote anti-termination.

For more information look in parts-registry, BBa_K374006

NutRsite followed by BioBrick terminator BBa_B0015

• BBa_K374007

This is a composite part, consisting of the Lambda nutR site part BBa_K374005 with the BioBrick terminator BBa_B0015 BBa_B0015 downstream of it

For more information look in parts-registry, BBa_K374007

Lambda N-gene with natural RBS

• BBa_K374011

This part contains the lambda N-gene which (also submitted as BioBrick BBa_K374006) with its natural RBS.

For more information look in parts-registry, BBa_K374011

Fluorescence Activated Cell Sorting (FACS) optimized GFP

• BBa_K374012

FACS optimized mutant of the Green Flourescent Protein. The protein contains the following three mutations: S65A, V68L and S72A. Excitation maximum at 488 nm.

For more information see US Patent 5,804,387 or parts-registry, BBa_K374012

Lambda N-gene followed by BioBrick RFP BBa_E1010

• BBa_K374013

This part is a composite part which contains the lambda N-gene with its natural RBS ( BBa_K374011 ) followed by a BioBrick RBS BBa_B0034 and RFP BBa_E1010 .

For more information look in parts-registry, BBa_K374013

Lambda nutR site followed by BioBrick terminator BBa_B1003

• BBa_K374014

Lambda nutR site BBa_K374005 which has the a BioBrick terminator BBa_B1003 downstream of it

For more information see BBa_K374014