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Team:Stockholm/21 June 2010
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Andreas
Preparation LB agar plates with antibiotics
- 100 ug/ml Amp (20 plates)
- 25 ug/ml Cm (20 plates)
Preparation of competent Top10 Escherichia coli cells
- Set a 5 ml LB ON culture; 37°C, 250 rpm.
Johan
Overnight culture of pEX
3 falcon tubes (50 ml), 10 ml LB in each.
24/12=2
2*30=60µl Amp
with tip from pEX glycerol stock
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