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From 2010.igem.org

Contents 1 10/5/2010 1.1 pTet GFP 1.2 Part:BBa_I732017 1.3 I13511 2 10/5/10

10/5/2010

pTet GFP

Untagged GFP behind a constitutive promoter. Usage and Biology

GFP only control Spring 2010 Distribution

2010 Kit Plate 2 8A pSB1A2

Part:BBa_I732017

Extracting DNA from I732017 using protocol from p 19 after extraction placing in freezer.

I13511

H.Y Electroporated a part I13511 with E-Coli,

After waiting 45 min, I streaked on an Amp/Agar plate and placed into incubator @ 37C overnight placing the rest of the spl. in incubator @ 37C.

--for JHull 5 October 2010 Rchamberlin 20:10, 17 October 2010 (UTC)

10/5/10

Suspension of A.tumefaciens and A.T9002 in LB amp

I’m suspending Agro Tumefaciens +T9002 in 20 ml of LB/Broth w/ 20 ul of Amp. in the A.T9002 suspension only 1- first I poured 20 ml of LB Broth (prepared by JH) into 6 - .45 ml steril tubes

2- I then added 20 ul of Amp into the tube, (only one containing Agro T9002) I pulled a sample of Agro + T9002 from a colony and suspended it into my LB/Amp Broth (plate streaked by IF)

3- I pulled cells from the colony A.Tume. and suspended them in LB Broth (20 ml)

4- Next, I pulled cells from a colony B.Thurlgensis (made by AM) and suspended in 20 ml of LB Broth

5- I pulled cells from plate containing B. Magetarium and suspended in Broth.

--for DGarvey 5 October 2010 Rchamberlin 16:41, 27 October 2010 (UTC)