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Team:Stockholm/9 July 2010
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Andreas
Plasmid preparations
Made plasmid preps from ON cultures.
DNA concentrations
| Sample | DNA conc (ng/μl) | A260/A280 |
| pSB1A3.BBa_J04450 (a) | ||
| pSB1A3.BBa_J04450 (b) | ||
| pSB1C3.BBa_J04450 (a) | ||
| pSB1C3.BBa_J04450 (b) | ||
| pSB1K3.BBa_J04450 (a) | ||
| pSB1K3.BBa_J04450 (b) | ||
| pEX (a) | ||
| pEX (b) | ||
| pMA.BBa_J18930 (a) | ||
| pMA.BBa_J18930 (b) | ||
| pMA.BBa_J18931 (a) | ||
| pMA.BBa_J18931 (b) | ||
| pMA.BBa_J18932 (a) | ||
| pMA.BBa_J18932 (b) |
Clonings
Two types of cloning were prepared:
- Transfer of the BBa_J1893x constructs (coding for fluorescent proteins) from pMA to pEX.
- pEX.BBa_J1893x will be good controls when testing IPTG-induced protein expression from pEX.
- Transfer of our pEX-carried parts to the pSB1x3 plasmids.
Digestions The following constructs were digested with XbaI and PstI:
- pSB1A3.BBa_J04450 (x)
- pSB1C3.BBa_J04450 (x)
- pSB1K3.BBa_J04450 (x)
- pEX (x)
- pMA.BBa_J18930 (x)
- pMA.BBa_J18931 (x)
- pMA.BBa_J18932 (x)
- pEX.SOD
- pEX.yCCS
Successful digestions were confirmed by loading 2 μ of each sample on a 1 % agarose gel:
