parts

	biobrick name	original name	description	resistance (bacteria)	resistance (plants)	sequence
1	BBa_K382000	pORE Open Series Vector with BioBrick Sites	Agrobacterium vector open series with biobrick cloning site	kan	pat	link
2	BBa_K382001	pORE Open Series Vector with BioBrick Sites	Agrobacterium vector open series with biobrick cloning site	kan	nptII	link
3	BBa_K382002	pORE Expression Series Vector with BioBrick Sites	Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site	kan	pat	link
4	BBa_K382003	pORE Expression Series Vector with BioBrick Sites	Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site	kan	nptII	link
5	BBa_K382004	pORE Reporter Series Vector with BioBrick Sites	Agrobacterium vector with gusA reporter and biobrick cloning site	kan	nptII	link
6	BBa_K382005	pORE Reporter Series Vector with BioBrick Sites	Agrobacterium vector with smgfp reporter and biobrick cloning site	kan	nptII	link
7	BBa_K382010	amiRNA LUT2	amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown	chloramphenicol	-	link
8	BBa_K382011	amiRNA BETA-OHASE 1	amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown	chloramphenicol	-	link
9	BBa_K382012	amiRNA LYC	amiRNA construct for lycopene beta cyclase (LYC) knockdown	chloramphenicol	-	link
10	BBa_K382014	ihpRNA Bet	Intron hairpin Bet Knockdown construct	amp	-	link
11	BBa_K382015	ihpRNA LTP	Intron hairpin LTP Knockdown construct	chloramphenicol	-	link
12	BBa_K382017	amiRNA LTP	LTP knockdown construct	amp	-	link
13	BBa_K382018	PDK intron hairpin	Intron hairpin construct	amp	-	link
14	BBa_K382019	ihpRNA Ger	Intron hairpin Ger Knockdown construct	chloramphenicol	-	link
15	BBa_K382020	Arabidopsis-optimized Brazzein	Sweet-tasting protein. 54 amino acid sequence.	chloramphenicol	-	link
16	BBa_K382021	Arabidopsis-optimized Miraculin	Taste-inverting protein turns sour to sweet. 191 amino acid sequence	chloramphenicol	-	link
17	BBa_K382022	pENTCUP2 promoter	Plant specific promoter. Used to drive expression of our flavor constructs in plants.	chloramphenicol	-	link
18	BBa_K382023	NOSt terminator	Plant specific terminator. Terminates transcription but no stop codon on end.	chloramphenicol	-	link
19	BBa_K382024	NOSt terminator + stop	Plant specific terminator. Terminates transcription with stop codon on 5' end.	chloramphenicol	-	link
20	BBa_K382025	Arabidopsis-optimized Miraculin StrepII N-terminus	Miraculin with StrepII tag on C-terminus for Western Blot.	chloramphenicol	-	link
21	BBa_K382026	Arabidopsis-optimized Brazzein StrepII N-terminus	Brazzein with StrepII tag on C-terminus for Western Blot.	chloramphenicol	-	link
22	BBa_K382027	Arabidopsis-optimized Miraculin StrepII C-terminus	Miraculin with StrepII tag on C-terminus for Western Blot.	chloramphenicol	-	link
23	BBa_K382028	Arabidopsis-optimized Brazzein StrepII C-terminus	Brazzein with StrepII tag on C-terminus for Western Blot.	chloramphenicol	-	link
24	BBa_K382030	RXRHm	Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.	chloramphenicol	-	link
25	BBa_K382031	RXRLc	Part of the Locust Retinoic Acid Receptor and VP16 activation domain fusion protein. RXRLc binds EcR in presence of Methoxyfenozide	amp	-	link
26	BBa_K382033	Ecdysone Receptor	Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide	chloramphenicol	-	link
27	BBa_K382034	Act2pLacO	Actin promoter with LacO sites	amp	-	link
28	BBa_K382035	LacIN	Lac Inhibitor with Nuclear Localization sequence - transcriptional inhibitor of the Act2LacO promoter	chloramphenicol	-	link
29	BBa_K382036	RXRHm	Human Retinoic Acid Receptor performs a function analogous to RXRLc. RXRHm has been shown to be sensitive to lower level of methoxyfenozide, but also to have higher background activation in the absence of methoxyfenozide.	amp	-	link
30	BBa_K382037	Ecdysone Receptor	Part of the fusion protein of Ecdysone receptor and Gal4 DNA binding domain. Ecdysone receptor binds RXR-VP16 in presence of Methoxyfenozide	amp	-	link
31	BBa_K382040	Arabidopsis-optimized Miraculin YFP2x C-terminus	Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression	chloramphenicol	-	link
32	BBa_K382041	Arabidopsis-optimized Brazzein YFP2x C-terminus	Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression	chloramphenicol	-	link
33	BBa_K382050	Arabidopsis-optimized Miraculin	Taste-inverting protein turns sour to sweet. 191 amino acid sequence	kan	pat	link
34	BBa_K382051	Arabidopsis-optimized Miraculin	Taste-inverting protein turns sour to sweet. 191 amino acid sequence	kan	nptII	link
35	BBa_K382052	Arabidopsis-optimized Brazzein	Sweet-tasting protein. 54 amino acid sequence.	kan	pat	link
36	BBa_K382053	Arabidopsis-optimized Brazzein	Sweet-tasting protein. 54 amino acid sequence.	kan	nptII	link
37	BBa_K382054	amiRNA GFP	amiRNA construct for GFP knockdown	kan	pat	link
38	BBa_K382055	amiRNA GFP	amiRNA construct for GFP knockdown	kan	nptII	link
39	BBa_K382058	amiRNA LUT2	amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown	kan	pat	link
40	BBa_K382059	amiRNA BETA-OHASE 1	amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown	kan	pat	link
41	BBa_K382060	amiRNA LYC	amiRNA construct for lycopene beta cyclase (LYC) knockdown	kan	pat	link
42	BBa_K382061	amiRNA LUT2	amiRNA construct for lycopene epsilon cyclase (LUT2) knockdown	kan	nptII	link
43	BBa_K382062	amiRNA BETA-OHASE 1	amiRNA construct for carotene beta-ring hydroxylase (BETA-OHASE 1) knockdown	kan	nptII	link
44	BBa_K382063	amiRNA LYC	amiRNA construct for lycopene beta cyclase (LYC) knockdown	kan	nptII	link
45	BBa_K382064	amiRNA LTP	LTP knockdown construct	kan	pat	link
46	BBa_K382065	amiRNA LTP	LTP knockdown construct	kan	nptII	link
47	BBa_K382066	ihpRNA Ger	Intron hairpin Ger Knockdown construct	kan	pat	link
48	BBa_K382067	ihpRNA Ger	Intron hairpin Ger Knockdown construct	kan	nptII	link
49	BBa_K382068	ihpRNA BetV1	Intron hairpin Bet Knockdown construct	kan	pat	link
50	BBa_K382069	ihpRNA BetV1	Intron hairpin Bet Knockdown construct	kan	nptII	link
51	BBa_K382070	ihpRNA LTP	Intron hairpin LTP Knockdown construct	kan	pat	link
52	BBa_K382071	ihpRNA LTP	Intron hairpin LTP Knockdown construct	kan	nptII	link

Team:Harvard/parts

From 2010.igem.org

parts