Team:Tsinghua/Notebook/29 September 2010

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Module I, Group 2c

Conduct PCR to amplify eGFP, mCherry, Kan and Chlr with DNA polymerase SuperMix.

PCR system (SuperMix): 

 H2O	        8.5μl
 primer1	0.5μl
 primer2	0.5μl
 template	0.5μl
 SuperMix	10μl
 Total	        20μl

Then, gel purification kit is used to purify the PCR product.

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