"
Team:Groningen/30 August 2010
From 2010.igem.org
Hydrophobofilm
pushing coatings into a greener future
Week 35
SDS-page - Chaplin ladder - Peter
Various biobricks where transformed in order to have them ready for eventual further experiments
The following Biobricks where transformed into the pNZ8901 backbone
Fluorescent proteins: [http://partsregistry.org/Part:BBa_E0240 GFP], [http://partsregistry.org/Part:BBa_E0422 Cyan], [http://partsregistry.org/Part:BBa_I13001 Yellow]
Promoters: [http://partsregistry.org/Part:BBa_I0500 pBad/araC promoter], [http://partsregistry.org/Part:BBa_R0010 lacI regulated promoter]
Modellers:
Write pieces about modelling for the wiki, do some more literature research and modelling. Joël, Laura, Djoke
Start writing information standard Arend
Find information about the killswitch system and write an introduction for the wiki about killswitches. Laura
Geeske
Left Groningen for internship, so no more labwork, but mentally supported the rest of the team that contuinued.
Jorrit
Colonies visible with possible pNZ-CCH1 or pNZ-dCS. 8 Colonies were grown overnight in LB medium. The cultures were miniprepped and checked on gel. On gel neither of the products was visible and nanodrop revealed low concentrations in the miniprep.
