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Team:Kyoto/Notebook3
From 2010.igem.org
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Notebook3
Measurement of Lysis Cassette
9/26 Wataru,Ken
After overnight pre-culture, dilute with supplemented M9 media as OD550 was 0.22, and measure OD550 every 30min. At the point of 30min, we add IPTG and continued to measure OD550. We used test tubes to cultivate E.coli. We cultivated E.coli in test tubes at 37 degreees.
1
Discussion We can observe the cell lysis and this result suggest that the timing of the start of cell lysis is depending on the concentration of IPTG.
2
Discussion When the concentration of IPTG is over 0.03mM, cell lysis occurs. We continued to incubate these cultures overnight.
9/27 Wataru Ken
All of the cultures was over 2.0 of OD550. We caouln't find out the cause of it.
10/4 Ken
3 We couln't observed the cell lysis.
10/8 Ken
4 In 1mM IPTG, the cell lysis surely occured, however, E.coli started to grow soony.
10/9 Ken
5 We considered that
