BioBricks submitted

BBa_K374008

Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogR

This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR^Gifsy1 and pRM^Gifsy1. Downstream of the pRM^Gifsy1 promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM^Gifsy1 and a repressor of the pR^Gifsy1.

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K374010 hence allowing expression of your inserted BioBrick.

This part has been characterized, for more information look here or in parts-registry, BBa_K374008

BBa_K374009

This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.

Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli.