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From 2010.igem.org

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Media:Obtaining Deionized Water in the ERB

Media:ERB Spectrophotometer

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol