Team:Queens-Canada/24 August 2010

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August 24, 2010

Glycerol Stock

Yuli

NpHR::eCFP, unc-54 3'UTR, pGpd-2, ChR2::eYFP::unc-54 3'UTR::1T3, eCFP::unc-54 3'UTR::1T3

Composition: 270µL 80% glycerol, 480 µL ddH2O, 750 µL cell culture.

Ligation

Yuli

pMec-7::1A3, pGpd-2::1C3, pHsp-3::1C3, pSra-10::1C3, pOdr-1::1C3, pOsm-10::1C3, pStr-1::1C3, eCFP::unc-54 3'UTR::1T3, pSB1A3, pSB1C3

Used T4 ligase. Products transformed into TOP10.

eYFP, eCFP, NpHR, pMec-7, pStr-1, pOsm-10, unc-54 3'UTR, pSra-10, eGFP, ChR2::eYFP::1C3, pHsp-3, pGpd-2, pOdr-1, NpHR::eCFP, pStr-220, eCFP::unc-54 3'UTR

Liquid Culture

Chris

pCeh-12::1C3, pRab-7::1C3, ChR2::eYFP::unc-54 3'UTR::1C3, pOdr-10::1C3