Team:Queens-Canada/23 August 2010

Digestion

Yuli

pSra-10::1C3, ChR2::eYFP::unc-54 3'UTR::1C3

Cut with NotI.

Gel Electrophoresis

Yuli

pSip-1, pRab-7, mCherry, pFlp-1, pOdr-10, pSra-10::1C3 (NotI digest), ChR2::eYFP::unc-54 3'UTR::1T3 (NotI digest)

Ligation

Yuli

pSip-1, pRab-7, mCherry, pFlp-1, pOdr-10, ChR2::eYFP::unc-54 3'UTR, pSB1C3

Backbone treated with CIAP for 30 mins. Used T4 ligase.

Heat Shock

Yuli Ligation products from 23/08/10

Digestion

pMec-7, pGpd-2, pHsp-3, pSra-10, pOdr-1, pOsm-10, pStr-1, eCFP::unc-54 3'UTR::1T3, pSB1A3

pStr-1::1C3, pMec-7::1A3, pOdr-1::1C3, pOsm-10::1C3, pHsp-3::1C3, pSra-10::1C3 cut wih EcoRI and SpeI.

eCFP::unc-54 3'UTR::1T3 cut with XbaI and PstI. Backbone treated with CIAP.