Team:Queens-Canada/19 August 2010
From 2010.igem.org
August 19, 2010
Minprep
Yuli and Thai
pHsp-3::1C3, pStr-220::1C3, pMec-7::1C3, eYFP::1C3, pSra-10::1C3
Digestion
Yuli and Chris
pHsp-3, pStr-220, pMec-7, eYFP, pSra-10
Cut with EcoRI and PstI.
Gel Electrophoresis
Yuli and Chris
pHsp-3, pStr-220, pMec-7, eYFP, pSrb-6
Split into one 40-lane and one 28-lane gel. Used 1.4 g agarose in 140 mL TBE. Gels run for 40 mins at 100 V.
Liquid Culture
Chris and Yuli and Thai
NpHR::eCFP::1C3